Journal:

Article Title: Phylogenetic Analyses and Expression Studies Reveal Two Distinct Groups of Calreticulin Isoforms in Higher Plants 1

doi: 10.1104/pp.103.024943

Figure Lengend Snippet: Differences in N-linked glycosylation status among different CRT isoforms in Arabidopsis. A, Analysis of CRT protein sequences predicted three potential glycosylation sites in CRT1 and one in CRT2 and CRT3, respectively. Analysis was performed using MacVector 7.0 Software. B, Arabidopsis homogenate was analyzed by 10% (w/v) SDS-PAGE (15 μg protein lane–1), blotted, and immunostained with polyclonal antibodies against maize CRT (1:5,000 [v/v]). Bands (a–c) next to lanes correspond to CRTs with differences in glycosylation status. C, Arabidopsis homogenate treated with N-glycosidase F (PNGase F) for 5 to 60 min under native conditions. The panel to the right shows lane probed with polyclonal antibodies against Arabidopsis calnexin (CNX; 1:1,000 [v/v]).

Article Snippet: Multiple alignment of 18 CRT protein sequences was performed using ClustalW, the MacVector 7.0 software package.

Techniques: Software, SDS Page